FastQCFastQC Report
Tue 17 Oct 2023
HFFVKDRX3_n02_Donor5_top_trogo.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVKDRX3_n02_Donor5_top_trogo.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69178517
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCT5820450784.13667931042812No Hit
CGATCGATCCGACTCGGTGCCCCTTTTTCAAGTTGATAACGGACTAGCCT4728220.6834809714119775No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCG3937300.5691506801164876No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTA1903420.27514611219549556No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTATCCT1852310.26775798041464227No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAT1835570.2653381540399312No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT1835270.26529478797586825No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTCGCCT1823980.2636627784316336No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT1652180.23882847907826646No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTT1585810.22923446017207913No Hit
NGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCT1422630.2056462123927866No Hit
AGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCT1373110.19848792075146685No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACGAGCCT1371510.19825663507646454No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAATGGACTAGCCT1168620.1689281659507098No Hit
CGATCGATCCGACTCGGTGCCACTTTTTTAAGTTGATAACGGACTAGCCT1131680.16358835792909523No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAATTTGATAACGGACTAGCCT969420.140133099412929No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTT936770.13541342610741425No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCC903280.13057232782252326No Hit
CGATCGATCCGACTCGGTGCCACTTTTTAAAGTTGATAACGGACTAGCCT781640.11298883438047681No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)