FastQCFastQC Report
Tue 17 Oct 2023
HFFVKDRX3_n02_Donor5_top_TCF1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVKDRX3_n02_Donor5_top_TCF1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57269148
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAT4757717483.07644807287862No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAG13284272.3196206795323726No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT2726320.476053878084584No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTA2032890.35497123163068534No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT1832180.3199244381983821No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTCT1801850.3146283929350581No Hit
NCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAT1168100.20396671520239834No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTT842520.14711586070740917No Hit
TCGATCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT795360.1388810603573149No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCGTAT790340.13800449763981124No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTCGCCTTAT742900.12972080534531438No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCT740260.12925982415523277No Hit
TCGATCCGACTCGGTGCCACTTTTTAAAGTTGATAACGGACTAGCCTTAT721800.12603644810640452No Hit
TCGATCCGACTCGGTGCCCCTTTTTCAAGTTGATAACGGACTAGCCTTAT714060.12468493507184707No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTT705690.12322341516238376No Hit
TGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT705320.12315880795013748No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG693500.12109486943999935No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAATGGACTAGCCTTAT684830.11958096530439043No Hit
TCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTA596410.10414158771839944No Hit
TCGATCCGACTCGGTGCCACTGTTTCAAGTTGATAACGGACTAGCCTTAT584830.10211955658917782No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)