FastQCFastQC Report
Tue 17 Oct 2023
HFFVKDRX3_n02_Donor5_pre_trogo.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVKDRX3_n02_Donor5_pre_trogo.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52036967
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTT4325894283.1311748050189No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTG3749960.7206338524687651No Hit
AATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTT2378610.45710004581934993No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTA2016460.3875052902295401No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTCGCCTT1773230.3407635191343877No Hit
GATCGATCCGACTCGGTGCCCCTTTTTCAAGTTGATAACGGACTAGCCTT1607560.3089265367829758No Hit
TATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTT1564530.30065741533321877No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT1425720.2739821481140513No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAT1424850.27381495927693095No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT1398910.2688300415356644No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTATCCTT1205720.23170451114108936No Hit
NATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTT1077500.20706433562893856No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACGAGCCTT1053620.2024752903066007No Hit
CATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTT984620.18921548598326263No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCT844840.1623538128192598No Hit
GCTCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTT810030.15566433762367435No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAATGGACTAGCCTT763590.14673991280083637No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG760870.14621720747098885No Hit
GATCGATCCGACTCGGTGCCACTTTTTTAAGTTGATAACGGACTAGCCTT747520.14365172359103864No Hit
GATCGATCCGACTCGGTGCCACTTTTTAAAGTTGATAACGGACTAGCCTT671020.1289506361890769No Hit
GATCGATCCGACTCGGTGCCACTGTTTCAAGTTGATAACGGACTAGCCTT641240.12322778151155506No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGAGAACGGACTAGCCTT635640.12215162347951601No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTT621240.1193843599685585No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCC618940.1189423664911139No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAATTTGATAACGGACTAGCCTT538800.10354177636832677No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)