FastQCFastQC Report
Tue 17 Oct 2023
HFFVKDRX3_n02_Donor5_pre_TCF1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVKDRX3_n02_Donor5_pre_TCF1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61651868
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT4968990280.59756113148104No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATG24165833.9197238922265907No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT2758000.4473505977142493No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAT1989990.32277854095191405No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTA1645250.26686133824850206No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAGT1615960.2621104684127332No Hit
AGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT1559370.25293150890415844No Hit
NGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT1210660.19637036788569No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAATGGACTAGCCTTATT1076920.174677594521548No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTT1061770.17222024805477104No Hit
CGATCCGACTCGGTGCCACTTTGTCAAGTTGATAACGGACTAGCCTTATT999960.16219459887249482No Hit
CGATCCGACTCGGTGCCACTTTTTTAAGTTGATAACGGACTAGCCTTATT847440.13745568909607084No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCT835160.13546385974874273No Hit
CGATCCGACTCGGTGCCACTTTTCAAGTTGATAACGGACTAGCCTTATTT758730.12306683067575502No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGAGAACGGACTAGCCTTATT758410.12301492632794192No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGTCTTATT756190.12265483991498846No Hit
TCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTA743880.12065814453505286No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAGG736430.11944974643752887No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTT694960.11272326736312353No Hit
CGATCCGACTCGGTGCCACTTTTTAAAGTTGATAACGGACTAGCCTTATT681610.11055788285279532No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACGAGCCTTATT637680.1034323891045767No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)