FastQCFastQC Report
Tue 17 Oct 2023
HFFVKDRX3_n02_Donor5_bot_trogo.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVKDRX3_n02_Donor5_bot_trogo.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46726895
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCC3952315784.58331545462201No Hit
ACGATCGATCCGACTCGGTGCCCCTTTTTCAAGTTGATAACGGACTAGCC2815740.6025951435463451No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTATCC2625740.5619333362509964No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCA1736820.3716960007721463No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT1258100.2692453671488337No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCT1221780.2614725416700596No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT1156030.2474014162507481No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAT1142150.24443096422306684No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACGAGCC1103830.2362301197201312No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTA1095550.23445812096010232No Hit
NCGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCC970690.2077368932816957No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTCGCC896730.19190874976820094No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTTAAGTTGATAACGGACTAGCC855350.18305303615829813No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAATGGACTAGCC704730.15081892344869052No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTT636780.1362769771028013No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTT558370.11949649126054705No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTAAAGTTGATAACGGACTAGCC518550.11097463248949027No Hit
ACGATCGATCCGACTCGGTTCCACTTTTTCAAGTTGATAACGGACTAGCC507030.10850924291031963No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG478130.102324368011185No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)