FastQCFastQC Report
Tue 17 Oct 2023
HFFVKDRX3_n02_Donor5_bot_TCF1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVKDRX3_n02_Donor5_bot_TCF1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42249428
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTA3496126882.74968361701843No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTC8748552.0706907558606473No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAT2276600.5388475318529756No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT1572780.37226066113841827No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT1287790.3048064934748939No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAATGGACTAGCCTTA862220.20407850255392807No Hit
NTCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTA853110.20192226034397437No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCT824930.19525234755840953No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG732080.17327571866771782No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTT726860.17204019898210218No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTT723720.17129699365397327No Hit
ATCGATCCGACTCGGTGCCACTTTTTTAAGTTGATAACGGACTAGCCTTA717430.1698082161017659No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTCGCCTTA640720.15165175727349492No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACGAGCCTTA622880.14742921489966682No Hit
ATCGATCCGACTCGGTGCCACTGTTTCAAGTTGATAACGGACTAGCCTTA564570.13362784461839342No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGTCTTA555220.13141479690565278No Hit
ATCGATCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAT516650.12228567922860399No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCC498730.11804420168718024No Hit
TCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTA491280.1162808642048361No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTATCCTTA482160.11412225509893294No Hit
ATCGATCCGACTCGGTGCCACTTTTTAAAGTTGATAACGGACTAGCCTTA472010.11171985571023588No Hit
ATGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAT436880.10340495023979969No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)