FastQCFastQC Report
Tue 17 Oct 2023
HFFVKDRX3_n02_Donor4_top_trogo.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVKDRX3_n02_Donor4_top_trogo.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34383082
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAT2898474384.29943249415513No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAG6827441.9856975008813929No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT1379550.40122930224812303No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTCT1146490.3334459662458415No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT1045290.30401288633753076No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTA972080.2827204379176945No Hit
NCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAT710610.20667431732850475No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTCGCCTTAT612120.17802941574580197No Hit
TCGATCCGACTCGGTGCCACTTTTTTAAGTTGATAACGGACTAGCCTTAT575440.1673613784825921No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTT502290.14608638050538925No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAATGGACTAGCCTTAT487520.1417906632104708No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCT438860.1276383542347949No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTT428830.12472122190791389No Hit
TCGATCCGACTCGGTGCCACTTTTTAAAGTTGATAACGGACTAGCCTTAT413690.12031789355008955No Hit
TCGATCCGACTCGGTGCCCCTTTTTCAAGTTGATAACGGACTAGCCTTAT369810.1075558031708734No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)