FastQCFastQC Report
Tue 17 Oct 2023
HFFVKDRX3_n02_Donor4_top_TCF1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVKDRX3_n02_Donor4_top_TCF1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65474693
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTT5499071983.9877462273859No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTA5901510.9013421414591436No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTG4491480.6859871798100681No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT2730270.4169962278402741No Hit
ATCCGACTCGGTGCCACTTTTTCCAGTTGATAACGGACTAGCCTTATTTT2108240.32199310961259486No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT2092870.3196456377428146No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAT1916480.29270545796984493No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTA1499980.2290930940294749No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTGT1476990.22558181372457903No Hit
NTCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTT1356060.20711208222083605No Hit
TCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTA1218070.186036763853173No Hit
ATACGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTT1189730.18170837395144412No Hit
ATCCGACTCGGTGCCACTTTTTTAAGTTGATAACGGACTAGCCTTATTTT1106860.16905157539264828No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1029710.15726839681401789No Hit
ATCCGACTCGGTGCCACTTTTTCAATTTGATAACGGACTAGCCTTATTTT958160.1463405105236614No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAATGGACTAGCCTTATTTT884650.13511327193240907No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCT828620.12655576712669733No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAAAGGACTAGCCTTATTTT760350.11612883774804411No Hit
ATCCGACTCGGTGCCACTTTTCAAGTTGATAACGGACTAGCCTTATTTTA742270.11336746550304558No Hit
ATCAGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTT732290.11184321246072891No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)