FastQCFastQC Report
Tue 17 Oct 2023
HFFVKDRX3_n02_Donor4_pre_trogo.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVKDRX3_n02_Donor4_pre_trogo.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62530610
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT5229105383.62472875284601No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATG19215313.0729445946553215No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT2742940.4386555640509504No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAT2253160.3603291252076383No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTA1703010.27234821473834975No Hit
AGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT1409750.2254495838118323No Hit
NGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT1269770.20306374749902487No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAGT1210940.19365555525525818No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTT1153310.184439269023603No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCT894200.14300196335842558No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAATGGACTAGCCTTATT876440.14016175437917525No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTT867090.1386664867014731No Hit
CGATCCGACTCGGTGCCACTTTTTTAAGTTGATAACGGACTAGCCTTATT842580.13474680640409553No Hit
CGATCCGACTCGGTGCCACTTTGTCAAGTTGATAACGGACTAGCCTTATT749270.11982451474565815No Hit
TCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTA689900.11032996479644129No Hit
CGATCCGACTCGGTGCCACTTTTTAAAGTTGATAACGGACTAGCCTTATT686500.1097862310954587No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCC662510.10594971006999611No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)