FastQCFastQC Report
Tue 17 Oct 2023
HFFVKDRX3_n02_Donor4_pre_TCF1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVKDRX3_n02_Donor4_pre_TCF1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68687125
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT5630517281.97340040073013No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTG16686462.4293431993259293No Hit
AATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT3328150.4845376771847708No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATGT2722330.3963377416073245No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT2120660.30874199495174676No Hit
TATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT2113380.3076821165538665No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTT2034480.29619524765376337No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAT1915360.27885284178075587No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1438800.20947157127336455No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTA1436380.2091192490586846No Hit
NATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT1409730.2052393370664444No Hit
CATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT1259480.18336478634096273No Hit
GATCCGACTCGGTGCCACTTTTTTAAGTTGATAACGGACTAGCCTTATTT1218120.1773432793991596No Hit
GATCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTT1066520.15527218528945563No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAATGGACTAGCCTTATTT1053370.15335770713943842No Hit
GATCCGACTCGGTGCCACTTTTTCCAGTTGATAACGGACTAGCCTTATTT873040.12710387863809994No Hit
GCTCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT844240.12291095310802425No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCT763210.11111398242392588No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATGG753120.10964500261147923No Hit
TCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTA752910.10961442919615577No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTCGCCTTATTT748760.10901024027428721No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGTCTTATTT734520.10693707153997202No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTT727880.10597036926498232No Hit
GATCCGACTCGGTGCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTT723300.10530357763554668No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)