FastQCFastQC Report
Tue 17 Oct 2023
HFFVKDRX3_n02_Donor4_bot_trogo.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVKDRX3_n02_Donor4_bot_trogo.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70570597
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTA5997307184.98308580271753No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTC12672181.7956741955860172No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAT2770090.3925274998027861No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT2111100.2991472496683002No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT1880430.2664608321224773No Hit
NTCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTA1471800.20855711338250404No Hit
ATCGATCCGACTCGGTGCCACTTTTTTAAGTTGATAACGGACTAGCCTTA1098400.15564555873035904No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCT1004210.14229864032466666No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAATGGACTAGCCTTA970770.13756012295035566No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG962210.13634715319185978No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTT953260.13507891962427354No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTT926230.13124871254808854No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTCGCCTTA923840.13091004458981692No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACGAGCCTTA884430.1253255658301998No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTATCCTTA835580.11840341948644703No Hit
ATCGATCCGACTCGGTGCCACTGTTTCAAGTTGATAACGGACTAGCCTTA789300.11184544747439219No Hit
ATCGATCCGACTCGGTGCCACTTTTTAAAGTTGATAACGGACTAGCCTTA722400.1023655786842784No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)