FastQCFastQC Report
Tue 17 Oct 2023
HFFVKDRX3_n02_Donor4_bot_TCF1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVKDRX3_n02_Donor4_bot_TCF1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40611255
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT3397758383.66543461904834No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTG7646401.882827802292739No Hit
AATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT2318240.5708368283619898No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT1550280.3817365407693015No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATGT1469690.3618922882338898No Hit
TATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT1453080.3578022890452413No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAT1367490.3367268507215549No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTT1068350.2630674673806559No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTA947230.23324322284548948No Hit
CATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT898750.221305645442378No Hit
NATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT850760.2094887242465174No Hit
GCTCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT639200.15739479117303812No Hit
TCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTA579260.14263533594320096No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTT572490.140968310385877No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCGTATTT551360.13576531924462812No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG548330.13501922065693367No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCT460470.11338482398537054No Hit
GATCCGACTCGGTGCCACTTTTCAAGTTGATAACGGACTAGCCTTATTTT417540.10281386280724396No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTGTTT411160.10124286974140544No Hit
GATCCGACTCGGTGCCACTTTTTCCAGTTGATAACGGACTAGCCTTATTT410650.1011172887910014No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)