FastQCFastQC Report
Tue 17 Oct 2023
HFFVKDRX3_n02_Donor2_pre_trogo.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVKDRX3_n02_Donor2_pre_trogo.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71491309
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTA6001156483.9424607542156No Hit
TCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTC12154281.7001059527389548No Hit
TCCGACTCGGTGCCACTTTTTCAATTTGATAACGGACTAGCCTTATTTTA5181710.7248027868674219No Hit
TCCGACTCGGTGCCACTTTTTCAAGTTGATAAAGGACTAGCCTTATTTTA2628500.3676670684544327No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT2215840.30994536692564967No Hit
TCCGACTCGGTGCCACTTTTTCCAGTTGATAACGGACTAGCCTTATTTTA2147070.30032601585180096No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT1916130.2680227886161659No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAT1657900.23190231416800608No Hit
NCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTA1477550.2066754715597668No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTT1337620.18710246304204614No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTA1317600.18430212265381798No Hit
TCCGACTCGGTGCCACTTTTTCAAGTTGATAACTGACTAGCCTTATTTTA1116640.15619241214341173No Hit
TCCGACTCGGTGCCACTTTTTTAAGTTGATAACGGACTAGCCTTATTTTA1019910.14266209617171788No Hit
TCCGACTCGGTGCCACTTTTTCAAGTTGATAATGGACTAGCCTTATTTTA977430.13672011516812485No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)