FastQCFastQC Report
Tue 17 Oct 2023
HFFVKDRX3_n01_Donor5_top_TCF1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVKDRX3_n01_Donor5_top_TCF1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57269148
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGACGAAAGGACGAAACACCGCTGGCTTCCCCTCT7240801.2643456822511137No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCTGGCTTCCCCTCT5228100.9128999090400297No Hit
CGATCATGATCGTCTTGTCGAAAGGACGAAACACCGCTGGCTTCCCCTCT4819620.8415735467201294No Hit
CGATCATGATCGTCTTGACCAAAGGACGAAACACCGCTGGCTTCCCCTCT4664990.8145729704237961No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACACCGCTGGCTTCCCCTCT4070540.7107736263162148No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAATCTCCGCTGGCTTCCCCTC2811390.4909082984786154No Hit
GATCGATAACGCATTTCAACTGGAAAGGACGTTTCACCGCTGGCTTCCCC2529710.4417230024096046No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGTTTCACCGCTGGCTTCCCC2527610.44135631282658505No Hit
ATCGATACCTTGGTTCTACAGGAAAGGACGAAACACCGCTGGCTTCCCCT2325190.40601092930525173No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGAAACACCGCTGGCTTCCCC2308460.4030896356271967No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGTATCACCGCTGGCTTCCCC1923460.3358632120736282No Hit
ATCGATACCTTGGTTCTACAGGAAAGGACGATTCTCCGCTGGCTTCCCCT1898600.33152230586702636No Hit
GATCGATAACGCATTTCAACTGGAAAGGACGAAACACCGCTGGCTTCCCC1845440.3222398209940193No Hit
CGATCATGATCGTCTTGTCCAAAGGACGAAACACCGCTGGCTTCCCCTCT1835330.32047447257291134No Hit
CGATCATGATCGTCTTGAGCAAAGGACGAAACACCGCTGGCTTCCCCTCT1828750.31932551187945035No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAATCTGCGCTGGCTTCCCCTC1813960.3167429695304704No Hit
TCGATCGTTACAATCTTCACGAAAGGACGAATCTCCGCTGGCTTCCCCTC1705100.29773448000309No Hit
GATCGATAACGCATTTCAACTGGAAAGGACGTATCACCGCTGGCTTCCCC1666980.2910781910008509No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGTAACACCGCTGGCTTCCCC1666310.290961199562459No Hit
CGATCATGATCGTCTTGTGCAAAGGACGAAACACCGCTGGCTTCCCCTCT1508480.263401858187239No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAAACACCGCTGGCTTCCCCTC1508400.26338788906026683No Hit
ATCGATTCCTTGGTTCTACAGGAAAGGACGAAACACCGCTGGCTTCCCCT1442910.2519524124926741No Hit
ATCGATTCCTTGGTTCTACAGGAAAGGACGATTCTCCGCTGGCTTCCCCT1385410.2419121024814268No Hit
TCGATCGTTACAATCTTCACGAAAGGACGAATCTGCGCTGGCTTCCCCTC1375210.24013103879247516No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGAAACACCGCTGGCTTCCCC1290300.2253045566523881No Hit
GATCGATAACGCATTTCAACTGGAAAGGACGTAACACCGCTGGCTTCCCC1241660.2168113274533087No Hit
CGATCATGATCTTCTTGACGAAAGGACGAAACACCGCTGGCTTCCCCTCT1218540.21277424975835157No Hit
ATCGATACCTTGGTTCTAGAGGAAAGGACGAAACACCGCTGGCTTCCCCT1206860.2107347572204147No Hit
CGATCATGATCTTCTTGACCAAAGGACGAAACACCGCTGGCTTCCCCTCT1167080.20378860883350317No Hit
ATCGATACCTTGGTTCTTGAGGAAAGGACGAAACACCGCTGGCTTCCCCT1124450.19634481029820802No Hit
TCGATCGTTACCATCTTGACGAAAGGACGAATCTCCGCTGGCTTCCCCTC1108520.1935632078898747No Hit
ATCGATACCTTGGTTCTTCAGGAAAGGACGAAACACCGCTGGCTTCCCCT1106340.19318254917988303No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACTCCGCTGGCTTCCCCTCT995360.1738038777877401No Hit
ATCGATTCCTTGGTTCTTGAGGAAAGGACGAAACACCGCTGGCTTCCCCT979580.17104846749247954No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGTTTCACCGCTGGCTTCCCC968870.16917835061908026No Hit
ATCGATTCCTTGGTTCTTCAGGAAAGGACGAAACACCGCTGGCTTCCCCT885510.15462252031407908No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGTAACACCGCTGGCTTCCCC856390.14953775809620914No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGTATCACCGCTGGCTTCCCC842530.14711760684828068No Hit
ATCGATTCCTTGGTTCTAGAGGAAAGGACGAAACACCGCTGGCTTCCCCT820570.14328308149441998No Hit
TCGATCGTTACAATCTTCACGAAAGGACGAAACACCGCTGGCTTCCCCTC805480.14064815491929442No Hit
ATCGATACCTTGGTTCTAGAGGAAAGGACGATTCTCCGCTGGCTTCCCCT795880.138971859682634No Hit
TCGATCGTTACCATCTTGACGAAAGGACGAAACACCGCTGGCTTCCCCTC793210.13850564006993785No Hit
GATCGATAACGCATTTCTAGTGGAAAGGACGAAACACCGCTGGCTTCCCC745690.1302079786484688No Hit
ATCGATACCTTGGTTCTACAGGAAAGGACGATACACCGCTGGCTTCCCCT677400.11828358263685013No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCTGGCTTCCCC675090.1178802240955287No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAATCACCGCTGGCTTCCCCTC672660.11745591186374904No Hit
TCGATCGTTACCATCTTCTCGAAAGGACGAATCTCCGCTGGCTTCCCCTC669540.11691111591183442No Hit
CGATCATGATCGTCTTGACCAAAGGACGAAACTCCGCTGGCTTCCCCTCT660470.11532736614136463No Hit
GATCGATAACGAATTTCAACTGGAAAGGACGTTTCACCGCTGGCTTCCCC655740.11450144150913508No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAATGTGCGCTGGCTTCCCCTC643350.11233797296932024No Hit
ATCGATACCTTGGTTCTACAGGAAAGGACGATACTCCGCTGGCTTCCCCT634650.11081883041109675No Hit
ATCGATTCCTTGGTTCTTCAGGAAAGGACGATTCTCCGCTGGCTTCCCCT627190.10951620932094187No Hit
ATCGATACCTTGGTTCTACAGGAAAGGACGATTGTCCGCTGGCTTCCCCT619740.10821533437165853No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGTTACACCGCTGGCTTCCCC619520.10817691927248509No Hit
ATCGATACCTTGGTTCTTCAGGAAAGGACGATTCTCCGCTGGCTTCCCCT590020.10302580370149736No Hit
ATCGATTCCTTGGTTCTAGAGGAAAGGACGATTCTCCGCTGGCTTCCCCT589700.10296992719360867No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)