FastQCFastQC Report
Tue 17 Oct 2023
HFFVKDRX3_n01_Donor4_bot_TCF1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVKDRX3_n01_Donor4_bot_TCF1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40611255
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGACGAAAGGACGAAACACCGCTGGCTTCCCCTCT5381471.3251178768053338No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCTGGCTTCCCCTCT4371851.0765119176937525No Hit
CGATCATGATCGTCTTGTCGAAAGGACGAAACACCGCTGGCTTCCCCTCT3809470.9380330649717671No Hit
GATCGATAACGCATTTCAACTGGAAAGGACGTTTCACCGCTGGCTTCCCC3578960.8812729377607267No Hit
CGATCATGATCGTCTTGACCAAAGGACGAAACACCGCTGGCTTCCCCTCT3359130.827142623393441No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGTTTCACCGCTGGCTTCCCC3358690.8270342790440729No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACACCGCTGGCTTCCCCTCT3229540.7952327501329373No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAAACACCGCTGGCTTCCCCTC2575630.634215810370795No Hit
ATCGATACCTTGGTTCTACAGGAAAGGACGAAACACCGCTGGCTTCCCCT1681140.41395913522002703No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAATCTCCGCTGGCTTCCCCTC1630200.40141581440908436No Hit
TCGATCGTTACCATCTTGACGAAAGGACGAAACACCGCTGGCTTCCCCTC1501570.36974232881992936No Hit
TCGATCGTTACAATCTTCACGAAAGGACGAAACACCGCTGGCTTCCCCTC1484150.36545287753358024No Hit
ATCGATACCTTGGTTCTACAGGAAAGGACGATTCTCCGCTGGCTTCCCCT1482530.3650539733381793No Hit
CGATCATGATCGTCTTGAGCAAAGGACGAAACACCGCTGGCTTCCCCTCT1391220.34257005847270666No Hit
CGATCATGATCGTCTTGTCCAAAGGACGAAACACCGCTGGCTTCCCCTCT1386720.3414619912632594No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGTTTCACCGCTGGCTTCCCC1253490.30865581474889164No Hit
CGATCATGATCGTCTTGTGCAAAGGACGAAACACCGCTGGCTTCCCCTCT1197320.29482467360341363No Hit
CGATCATGATCGTCTTGACGAAAGGACGAAACTCCGCTGGCTTCCCCTCT1128720.27793280458828473No Hit
ATCGATTCCTTGGTTCTACAGGAAAGGACGATTCTCCGCTGGCTTCCCCT1079040.2656997425959872No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGTATCACCGCTGGCTTCCCC1053530.2594182327042097No Hit
TCGATCGTTACAATCTTCACGAAAGGACGAATCTCCGCTGGCTTCCCCTC1031180.2539148322306218No Hit
ATCGATTCCTTGGTTCTACAGGAAAGGACGAAACACCGCTGGCTTCCCCT1020160.25120129875326436No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGAAACACCGCTGGCTTCCCC999280.2460598669014292No Hit
TCGATCGTTACCATCTTGTCGAAAGGACGAAACACCGCTGGCTTCCCCTC992380.24436083051361007No Hit
GATCGATAACGAATTTCAACTGGAAAGGACGTTTCACCGCTGGCTTCCCC982670.24196986771278062No Hit
GATCGATAACGCATTTCAACTGGAAAGGACGTATCACCGCTGGCTTCCCC944900.23266949026815348No Hit
ATCGATACCTTGGTTCTAGAGGAAAGGACGAAACACCGCTGGCTTCCCCT926040.22802545747478128No Hit
GATCGATAACGCATTTCAACTGGAAAGGACGAAACACCGCTGGCTTCCCC903470.2224678848265093No Hit
ATCGATACCTTGGTTCTTGAGGAAAGGACGAAACACCGCTGGCTTCCCCT881940.2171663988221984No Hit
CGATCATGATCTTCTTGACGAAAGGACGAAACACCGCTGGCTTCCCCTCT866850.21345068011318535No Hit
ATCGATACCTTGGTTCTTCAGGAAAGGACGAAACACCGCTGGCTTCCCCT833700.20528791833692409No Hit
TCGATCGTTACCATCTTCTCGAAAGGACGAAACACCGCTGGCTTCCCCTC830740.20455905635026547No Hit
CGATCATGATCTTCTTGACCAAAGGACGAAACACCGCTGGCTTCCCCTCT824720.20307670866118271No Hit
GATCGATAACGCATTTCAACTGGAAAGGACGTTTGACCGCTGGCTTCCCC814530.20056755202467885No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAATCACCGCTGGCTTCCCCTC806360.19855579444663798No Hit
ATCGATTCCTTGGTTCTTGAGGAAAGGACGAAACACCGCTGGCTTCCCCT757230.18645816289105077No Hit
GATCGATAACGCATTTCTAGTGGAAAGGACGTTTCACCGCTGGCTTCCCC747360.1840278021449965No Hit
GATCGATAACGCATTTCATCTGGAAAGGACGTTTCACCGCTGGCTTCCCC733960.1807282242324203No Hit
TCGATCGTTACCATCTTGACGAAAGGACGAATCTCCGCTGGCTTCCCCTC709970.17482099482027827No Hit
CGATCATGATCGTCTTGACCAAAGGACGAAACTCCGCTGGCTTCCCCTCT705380.1736907662666421No Hit
ATCGATTCCTTGGTTCTTCAGGAAAGGACGAAACACCGCTGGCTTCCCCT659860.16248205085018919No Hit
ATCGATACCTTGGTTCTAGAGGAAAGGACGATTCTCCGCTGGCTTCCCCT653310.16086919746754932No Hit
CGATCATGATCGTCTTGTCGAAAGGACGAAACTCCGCTGGCTTCCCCTCT652380.16064019691093023No Hit
ATCGATTCCTTGGTTCTAGAGGAAAGGACGAAACACCGCTGGCTTCCCCT614480.15130780863580798No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACTCCGCTGGCTTCCCCTCT600970.14798114463588974No Hit
TCGATCGTTACCATCTTCACGAAAGGACGAAACTCCGCTGGCTTCCCCTC599910.1477201332487755No Hit
CGATCATGATCGTCTTGAGGAAAGGACGAAACTCCGCTGGCTTCCCCTCT576500.14195572138807333No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGTAACACCGCTGGCTTCCCC575270.14165284968415776No Hit
ATCGATACCTTGGTTCTACAGGAAAGGACGATACTCCGCTGGCTTCCCCT573090.14111605268046998No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCTGGCTTCCCCTC527820.1299688965534308No Hit
ATCGATACCTTGGTTCTACAGGAAAGGACGAAACTCCGCTGGCTTCCCCT517910.12752868632107034No Hit
ATCGATTCCTTGGTTCTTCAGGAAAGGACGATTCTCCGCTGGCTTCCCCT517680.12747205177480972No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGAAACACCGCTGGCTTCCCC515610.126962340858464No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGTTTGACCGCTGGCTTCCCC509300.12540858439366132No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGTTTCACCGCTGGCTTCCCC503020.1238622150435883No Hit
GATCGATAACGAATTTCTACTGGAAAGGACGTTTCACCGCTGGCTTCCCC497770.12256946996589985No Hit
TCGATCGTTACAATCTTCACGAAAGGACGAATCACCGCTGGCTTCCCCTC496800.12233061992297455No Hit
ATCGATACCTTGGTTCTACAGGAAAGGACGATTGTCCGCTGGCTTCCCCT493160.12143431666911057No Hit
ATCGATTCCTTGGTTCTAGAGGAAAGGACGATTCTCCGCTGGCTTCCCCT492150.12118561713987909No Hit
GATCGATAACGCATTTCAACTGGAAAGGACGTAACACCGCTGGCTTCCCC484570.11931913948485463No Hit
ATCGATACCTTGGTTCTTCAGGAAAGGACGATTCTCCGCTGGCTTCCCCT481710.11861490121396148No Hit
GATCGATAACGCATTTCTTCTGGAAAGGACGTATCACCGCTGGCTTCCCC472520.1163519817351126No Hit
TCGATCGTTACCATCTTGAGGAAAGGACGAAACACCGCTGGCTTCCCCTC465750.11468495617778865No Hit
ATCGATACCTTGGTTCTACAGGAAAGGACGATACACCGCTGGCTTCCCCT460480.11338728635694711No Hit
CGATCATGATCTTCTTGAGGAAAGGACGAAACACCGCTGGCTTCCCCTCT441860.10880235048141211No Hit
ATCGATTCCTTGGTTCTACAGGAAAGGACGATACTCCGCTGGCTTCCCCT441070.1086078231268647No Hit
ATCGATTCCTTGGTTCTACAGGAAAGGACGAAACTCCGCTGGCTTCCCCT434060.10688170065170358No Hit
ATCGATTCCTTGGTTCTTGAGGAAAGGACGATTCTCCGCTGGCTTCCCCT432350.10646063511211364No Hit
TCGATCGTTACAATCTTGACGAAAGGACGAAACACCGCTGGCTTCCCCTC430970.1061208278345498No Hit
TCGATCGTTACCATCTTGACGAAAGGACGAATCACCGCTGGCTTCCCCTC418930.1031561324563843No Hit
ATCGATACCTTTGTTCTACAGGAAAGGACGATTCTCCGCTGGCTTCCCCT416890.10265380865476824No Hit
GATCGATAACGCATTTCTACTGGAAAGGACGTTACACCGCTGGCTTCCCC407110.10024560925290292No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)