FastQCFastQC Report
Fri 1 Jul 2022
HFFVHBGXM_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVHBGXM_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619805156
Sequences flagged as poor quality0
Sequence length90
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20288730.3273404521339606No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA8304140.13397984704728724Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTAAACTCCATCTAAAACTAAATATAACCATGAGACCGATAGTAAAC7965220.1285116769825645No Hit
GTAAACTCCATCTAAAACTAAATATAACCATGAGACCGATAGTAAACAAG7679990.12390974688826241No Hit
GCTTGATAGTGCAGCACTAAGTGGGTGGTAAACTCCATCTAAAACTAAAT7313270.11799304877031387No Hit
ACTAAATATAACCATGAGACCGATAGTAAACAAGTACCGTGAGGGAAAGT7231930.11668070086206252No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAGTTTTTATTTATTAAT6413240.10347187237661508Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGAAAAGAACTCTGAATAGAGAGTTAAACAGTACGTGAAACTGCTTAG6247490.10079764486502593No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)