FastQCFastQC Report
Fri 1 Jul 2022
HFFVHBGXM_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVHBGXM_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619805156
Sequences flagged as poor quality0
Sequence length10
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTACGTA55793276190.01744428857252No Hit
AGATCTCGGT113304401.828064818486279Illumina Single End PCR Primer 1 (100% over 10bp)
GCATTACGTT89184731.4389155872075385No Hit
TCATTACGTA33463800.5399083837243845No Hit
TCATTACTTA19202280.30981155632722746No Hit
GGGGGGGGGG16162110.2607611415223529No Hit
AGATCTCGGG13143980.21206632233953215No Hit
TAATTACTTA11580610.1868427503045812No Hit
GCATTACGGA11477780.18518368053072473No Hit
TAATTAATTA11442600.18461608280005984No Hit
TCATTACGTT11233450.18124163523415415No Hit
GAATTACGTA10898400.17583590414662506No Hit
GCAGTACGTA9523120.15364699547610733No Hit
GCCTTACGTT8740680.14102302821114318No Hit
GCCTTACGTA8235360.1328701434681192No Hit
GCATGACGTA8125230.13109329474503437No Hit
TAATTACGTA7385800.11916325523435949No Hit
GCATTACGTC7034250.11349131145336906No Hit
GCATTCCGTA7004310.11300825642050645No Hit
GCATTACGTG6340490.10229811640998998No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)