FastQCFastQC Report
Fri 1 Jul 2022
HFFVHBGXM_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVHBGXM_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619805156
Sequences flagged as poor quality0
Sequence length10
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGCCATGA57797234393.25065101588152No Hit
GGGGGGGGGG206622873.33367459111618No Hit
AATGCCATGC23061630.37207870532784015No Hit
AATGCATGAA15604990.25177251026288655No Hit
ATGCCATGAA9286000.14982127705952802No Hit
AATGCCAGGA7340090.11842576540295835No Hit
AATGCCATAA7081540.11425429316693198No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)