FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49212989
Sequences flagged as poor quality0
Sequence length37
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28867205.865768486445723No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG771670.156802099543273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA12650.018.62147322
GACGTAG14050.016.87668223
TATCGCG44900.016.8427939
TCGACGT13950.016.77531821
CGTAGAA15300.016.3072225
ATCGCGG48150.015.93136810
ATATCGC48900.015.180468
GATATCG50550.014.7454317
CCCTACG13650.014.64736527
TCGCGGC54400.014.5000711
GGATATC58150.013.0592666
TGCCCTA16000.012.97867425
ACGTAGA18750.012.81184524
AAGGGGG85900.012.7149511
GGGATCC39700.012.3415323
TCCTACG12950.012.3253987
GGGGATC40250.012.1417532
CGCGGCC66450.012.05765112
GCCCTAC16950.011.88592126
CGGCCGC70000.011.53502914
Produced by FastQC (version 0.11.5)