FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n02_WT_g3_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n02_WT_g3_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27539528
Sequences flagged as poor quality0
Sequence length37
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3257301.1827726313973137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA26600.019.2294622
TCGACGT31400.016.33937621
TATCGCG131200.015.8182889
CCCTACG33100.015.78153727
ATCGCGG135850.015.29991310
GACGTAG34050.015.02218923
ATATCGC139400.015.0213598
TCGCGGC139200.015.01002911
GATATCG141300.014.7856877
CGTAGAA34650.014.67251725
ACGTTTG37700.014.22530731
CGCTCGA36450.013.99087918
CGCGGCC150900.013.92873112
CTCGACG38150.013.32631820
CGGCCGC158200.013.197988514
GCGGCCG160500.013.18264913
TAACGGT18000.012.91708828
TGCCCTA42300.012.605268525
GGATATC168950.012.5493866
GCCCTAC42550.012.53127526
Produced by FastQC (version 0.11.5)