FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n02_WT_g3_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n02_WT_g3_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36254935
Sequences flagged as poor quality0
Sequence length37
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1033280.2850039587714059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA30100.021.2678622
TCGACGT36850.017.58228921
GACGTAG39100.016.57072823
TATCGCG97150.016.4165639
CCCTACG36150.015.90814827
CGTAGAA40850.015.89865725
TCCTACG46550.015.5153927
CGCTCGA41150.015.36853318
ATCGCGG104250.015.2987610
TCGCGGC106100.015.12009111
GGGGATC141750.014.925522
ATATCGC109350.014.7836558
CTCGACG44200.014.44794920
GGGGGAT177500.014.4108421
CGCTAAT46400.014.36464624
ACGTTTG41200.013.95735631
GATATCG117000.013.9223777
GCTCGTA55450.013.641499529
GCTCGAC46750.013.62687619
CGCGGCC119650.013.394999512
Produced by FastQC (version 0.11.5)