FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n02_WT_g2_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n02_WT_g2_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22246816
Sequences flagged as poor quality0
Sequence length37
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2304601.0359235227189365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA21950.019.56029722
TCGACGT26550.016.11290221
CCCTACG26750.015.93520427
TATCGCG108450.015.4208179
GACGTAG28150.015.4174223
ATCGCGG112100.014.97415210
CGTAGAA30700.014.74265225
TCGCGGC114600.014.67477311
ACGTTTG29200.014.54433831
ATATCGC117000.014.5060658
GATATCG118300.014.3197467
CGCGGCC122650.013.77510512
CGCGTCT22150.013.64585618
CTAAACG13000.013.23456822
CGCTCGA32450.013.13584218
TGCCCTA33800.012.97779425
CGGCCGC129200.012.95701714
GCGGCCG132150.012.84374713
ACGATCG3200.012.59359231
CTCGACG33800.012.56484620
Produced by FastQC (version 0.11.5)