FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n02_WT_g2_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n02_WT_g2_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46292421
Sequences flagged as poor quality0
Sequence length37
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1799490.3887223785509079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA33700.018.62789222
TCGACGT40450.015.78748821
TATCGCG62250.014.86439
TCCTACG44650.014.5095997
CGTAGAA44600.014.38810425
GACGTAG44300.014.34570923
CGCTCGA45500.013.83113918
CACGTAG27200.013.27818727
ATCGCGG70750.013.12242410
TCGCGGC73950.012.70157411
ATATCGC75100.012.67200858
CTCGACG49850.012.49941320
ACACGTA30450.012.36969526
GATATCG79550.012.1185537
GCTCGAC52650.011.98205619
GGGGGAT159800.011.757771
GCTCGTA56150.011.59416729
CCGCTCG55250.011.39034917
TACGTTC58750.010.86933710
GGGGATC119250.010.8010792
Produced by FastQC (version 0.11.5)