FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n02_WT_g1_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n02_WT_g1_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22872461
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15175696.634917860391148No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT985390.43081940329901536No Hit
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG965080.4219397291791207No Hit
CTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA632980.2767432852984206No Hit
CTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTA627800.27447855305119984No Hit
GAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG603360.263793214031494No Hit
CTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC510950.2233909153894721No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG421190.18414721529091252No Hit
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAA393130.17187918694013732No Hit
GATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGG379770.1660381014530968No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG371470.16240928337357313No Hit
CTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATT357770.15641954750737141No Hit
GATCCTAGAGACCATTCGCGATTCCATGAGACTCCAA304430.1330989262589627No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG293540.12833774205582862No Hit
GATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGAC276950.12108447796675662No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG274700.12010076222230744No Hit
AAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG272750.11924820857711813No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG252420.1103597903172728No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG229390.10029091316408846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTG125250.024.72548131
TAGGGGG110850.024.7252141
ACGGTTG52500.024.1206430
CGGTTGG53200.024.09466431
AGCTAGC70050.023.63143531
TAACGGT57300.022.72311228
ACTGGAT160150.022.62171
CCCTACG139400.022.23875227
AACGGTT59550.021.86412629
CGACGTA145750.021.72682622
ACTCAAT147300.021.53982231
TGCCCTA145500.021.42307325
GACGTAG148100.021.41342423
GCCCTAC145350.021.41323726
ATGCATC168950.021.181736
CGTAGAA150800.021.0915625
TCGCGCT20500.020.94367631
ACGTAGA151300.020.92988824
CTAAACG54100.020.85790322
TTCGCGC20450.020.8432530
Produced by FastQC (version 0.11.5)