FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n02_WT_g1_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n02_WT_g1_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42510062
Sequences flagged as poor quality0
Sequence length37
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG872260.20518906794349065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA39200.021.07546222
TCGACGT47400.017.4622621
TATCGCG119550.017.1001559
GACGTAG51200.016.28736523
ATCGCGG126350.016.22925610
TCGCGGC129650.015.82857111
ATATCGC132500.015.698218
TCCTACG57050.015.5393917
CGTAGAA54800.015.471868525
CCCTACG41950.015.408360527
CGCTCGA55350.015.06635918
GGGGATC157400.014.879562
GATATCG140400.014.8473447
GGGGGAT192450.014.6367111
CGCGGCC146500.014.15638712
CTCGACG58600.014.1244820
CGCTAAT51200.013.95642324
ACGTTTG49150.013.52895231
CGGCCGC154250.013.43539314
GGGATCC165700.013.4320683
Produced by FastQC (version 0.11.5)