FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n02_EZH2KO_g3_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n02_EZH2KO_g3_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17894473
Sequences flagged as poor quality0
Sequence length37
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4167902.3291549295695937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA12300.017.1381822
TAGGGGG36300.016.1852461
TATCGCG56900.014.627419
TCGACGT14350.014.47400421
ATCGCGG59500.014.06653410
TCGCGGC61950.013.56060911
CGCTCGA15250.013.51838718
ATATCGC63050.013.3730418
GACGTAG15600.013.31437723
GATATCG63950.013.1115027
CGTAGAA16500.012.68204425
CGCGGCC70350.012.33811412
CTCGACG16750.012.30737120
CCCTACG15750.012.10545127
GCTCGAC17650.011.76794719
CGTGCAA14400.011.73304429
CGGCCGC74050.011.47092814
TAACGGT10150.011.45378928
GCGGCCG76750.011.35003913
GTTAACG10100.011.04976326
Produced by FastQC (version 0.11.5)