FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n02_EZH2KO_g3_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n02_EZH2KO_g3_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36907920
Sequences flagged as poor quality0
Sequence length37
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1902600.5154991123856343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA47500.021.70034822
TCGACGT53750.019.26351221
CGTAGAA57700.018.10581225
GACGTAG58350.017.877723
TATCGCG118250.017.4717779
ATCGCGG125550.016.54251110
TCGCGGC125800.016.49769611
ATATCGC128350.016.3145758
GATATCG129900.016.0832187
CCCTACG43150.015.94974627
CGCTCGA64700.015.90764818
ACGTTTG44600.015.81279831
CTCGACG68100.015.18124420
CGTATAA50600.015.07144724
CGCGGCC141900.014.76822212
CACGTAG28400.014.7365927
TCCTACG49350.014.6977497
GGGGATC124050.014.3190092
GCTCGAC72550.014.27155219
CGGCCGC148350.014.10553514
Produced by FastQC (version 0.11.5)