FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n02_EZH2KO_g2_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n02_EZH2KO_g2_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24588020
Sequences flagged as poor quality0
Sequence length37
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7354032.990899633236023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA19750.019.46368222
TATCGCG95050.016.9750739
ATCGCGG99450.016.2087210
CCCTACG27400.015.89669327
ATATCGC103650.015.8059798
GATATCG104000.015.7221487
TCGCGGC103550.015.612102511
TCGACGT25150.015.2229421
GACGTAG25950.015.17188323
CGTAGAA26350.014.70617825
ACGTTTG29050.014.512876531
CGCGGCC115750.014.23464112
CTAAACG13150.014.14477322
GCGGCCG120250.013.7923513
CGCTCGA27900.013.77791518
TAGGGGG41250.013.5676341
CGGCCGC122350.013.46693814
CGCGTCT20500.013.45867118
GTTAACG12950.013.40562126
TAACGGT14100.012.97215728
Produced by FastQC (version 0.11.5)