FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n02_EZH2KO_g2_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n02_EZH2KO_g2_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36825954
Sequences flagged as poor quality0
Sequence length37
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2116830.5748201390790854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA48000.022.76584622
TCGACGT54650.020.052421
GACGTAG56950.019.35145223
CGTAGAA59000.018.81050725
TATCGCG122850.018.6720689
ATCGCGG129100.017.81630510
TCGCGGC131900.017.5443311
CCCTACG43900.017.19579327
ATATCGC134650.017.1511128
GATATCG138500.016.6735787
ACGTTTG45400.016.62667831
CGCTCGA65700.016.6092618
CGCGGCC144300.016.06930412
CTCGACG68050.016.0579420
CGGCCGC150450.015.49527314
GCTCGAC71050.015.40199919
GCGGCCG155250.015.2058513
ACGTAGA74350.014.96872124
CCGCTCG73250.014.93967817
CGCGTCT20400.014.81656218
Produced by FastQC (version 0.11.5)