FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n02_EZH2KO_g1_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n02_EZH2KO_g1_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24760515
Sequences flagged as poor quality0
Sequence length37
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11363044.589177567590981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA20750.019.34714722
TATCGCG102200.016.3486569
TCGACGT25250.015.96055821
ATCGCGG106350.015.75470410
GATATCG108200.015.5990837
ATATCGC110350.015.3940348
GACGTAG26900.015.3850423
TCGCGGC110950.015.14379111
CGTAGAA27350.015.13181225
CCCTACG27950.014.69642127
TAGGGGG49800.014.6307621
TAACGGT14650.014.07215728
CGCGGCC122150.013.83160812
CGCTCGA28850.013.75423418
CGGCCGC127150.013.36103214
CTAAACG15250.013.31486322
ACGTTTG30450.013.28531431
GGATATC128800.013.1884836
GCGGCCG132250.013.04503613
CTCGACG30750.012.85358220
Produced by FastQC (version 0.11.5)