FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n02_EZH2KO_g1_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n02_EZH2KO_g1_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33565195
Sequences flagged as poor quality0
Sequence length37
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1652620.49236120928241295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA30800.021.7907322
GACGTAG39400.017.30999423
TCGACGT38800.017.29777121
TCCTACG48350.016.8930457
TATCGCG110250.016.7576649
CGTAGAA41700.016.54097725
CCCTACG38550.016.08381327
ATCGCGG115100.016.05163810
TCGCGGC117400.015.84302111
GGGGATC137050.015.5054582
ATATCGC121600.015.3592718
ACGTTTG41950.014.92723831
CGCTCGA45000.014.81136318
GATATCG126400.014.6893847
CGCGTCT24900.014.50426418
CTCGACG46200.014.49327920
CGCGGCC129650.014.39404212
GGGGGAT179050.014.1217961
GGGATCC146350.014.1172273
CGCTAAT47950.013.90029924
Produced by FastQC (version 0.11.5)