FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49212989
Sequences flagged as poor quality0
Sequence length37
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18125503.683072369369802No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2527130.5135087405481508No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1806300.3670372470162298No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG825390.1677179169101068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA168250.030.108481
TCGGAAG182200.028.8960193
ATCGGAA183250.028.7752632
ACACGTC157950.028.35459513
GCACACG166100.028.08279611
CACACGT165750.027.97366312
AGGGGGG287450.027.9374351
CGGAAGA189250.027.7208164
ACGTCTG160150.027.18137215
CGTCTGA160750.027.08939216
AGAGCAC182850.027.0275158
CACGTCT164100.026.95203214
AGCACAC182600.026.26659610
GAACTCC171400.023.10258921
CTGAACT189550.022.44334819
TGAACTC189250.022.25777620
AACTCCA193550.020.92315722
TGGGGGG125500.020.9004231
CAGTCAC172600.020.06242227
CTCCAGT189300.019.98683524
Produced by FastQC (version 0.11.5)