FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n01_WT_g3_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n01_WT_g3_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27539528
Sequences flagged as poor quality0
Sequence length37
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGT6664622.4200196895168284TruSeq Adapter, Index 15 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2620330.9514796332021377No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1514280.5498569183901773No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG834170.30289916370389497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA693300.030.5766161
ACACGTC698600.030.39516413
ACGTCTG701600.030.17230215
TCGGAAG707950.030.077033
GCACACG706850.030.07543611
CACACGT708200.030.0004912
CACGTCT706800.029.97899414
CGTCTGA707800.029.91446916
ATCGGAA711950.029.8886152
CGGAAGA724500.029.4135574
AGAGCAC731500.029.1406218
GAGCACA736150.028.9668129
AGCACAC735600.028.94859510
TCACATG732850.028.57352430
CAGTCAC741500.028.42659827
CACATGT737400.028.41198231
GAACTCC744250.028.40553721
GTCACAT743600.028.26883729
TCCAGTC747250.028.181225
GTCTGAA753600.028.17466417
Produced by FastQC (version 0.11.5)