FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n01_WT_g3_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n01_WT_g3_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36254935
Sequences flagged as poor quality0
Sequence length37
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG855940.23608923860986095No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG853170.23532520469282317TruSeq Adapter, Index 5 (100% over 37bp)
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCG509090.14041950426886712No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG441030.12164688752027827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA101950.027.7378751
ACACGTC106500.026.73455213
ACGTCTG114650.024.739415
CGTCTGA114300.024.73372816
CACACGT118150.024.12453812
ATCGGAA118850.023.9454062
GCACACG121250.023.66123611
TCGGAAG121100.023.6411743
CACGTCT123750.022.99545714
CGACGTA29150.020.47202122
AGAGCAC145500.020.0693118
CGGAAGA146400.019.58734
GTCACAC145600.019.48087729
GAGCACA155150.018.8408419
AGCACAC158000.018.52068710
TCGACGT32900.018.23283621
GAACTCC162750.017.68601621
CTAGTAC96450.017.435649
TATCGCG79500.017.1953839
ATCGCGG80100.017.06664810
Produced by FastQC (version 0.11.5)