FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n01_WT_g2_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n01_WT_g2_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22246816
Sequences flagged as poor quality0
Sequence length37
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTT3678491.6534905489396776TruSeq Adapter, Index 8 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1955480.8789932006449822No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG909560.4088495180613711No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG671480.3018319565370613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA393750.030.4127981
ACACGTC397550.030.18306413
ACGTCTG400000.029.94761815
TCGGAAG403850.029.7806023
CGTCTGA403300.029.72172416
CACACGT403700.029.71557212
GCACACG404800.029.68882811
ATCGGAA405500.029.6402442
CACGTCT405150.029.5976814
CGGAAGA418800.028.7544634
AGAGCAC425000.028.3472168
AGCACAC425750.028.31177910
GAGCACA429450.028.0859629
CAGTCAC431850.027.69418327
GAACTCC434350.027.57164421
TCCAGTC438250.027.25840625
GTCTGAA442450.027.14709717
CACAGTT443600.026.91140631
CTGAACT450100.026.77867919
CCAGTCA446600.026.74882126
Produced by FastQC (version 0.11.5)