FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n01_WT_g2_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n01_WT_g2_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46292421
Sequences flagged as poor quality0
Sequence length37
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC1092730.23604943884874804TruSeq Adapter, Index 4 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG912320.19707761665781098No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG597250.12901679953182835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA128800.027.6239571
ACACGTC133900.026.6926813
ACGTCTG145700.024.5096115
CGTCTGA147050.024.3054716
CACACGT149100.023.99205212
TCGGAAG150350.023.8875053
GCACACG152050.023.65926611
ATCGGAA152300.023.5003132
CACGTCT155550.023.02731914
AGAGCAC184200.019.841238
CGGAAGA183350.019.7487094
CGACGTA30000.019.22033522
GAGCACA196500.018.6938119
AGCACAC198800.018.43865210
GAACTCC199750.018.06493621
TATCGCG50900.017.7830899
CACTGAC203650.017.42898431
TCGACGT33450.017.19166621
AGTCACT214150.016.8710628
ATCGCGG53750.016.86909710
Produced by FastQC (version 0.11.5)