FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n01_WT_g1_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n01_WT_g1_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22872461
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14541676.357719879815294No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTC9063323.9625469248805363TruSeq Adapter, Index 13 (100% over 37bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8904223.8929872915730406No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2072100.9059366195880714No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCG1195140.5225235710315562No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT909050.39744302110734825No Hit
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG885360.3870855873357921No Hit
CTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTA729340.3188725515806979No Hit
GATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGG623940.2727909340407226No Hit
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAA524710.22940688367552578No Hit
CTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA506170.22130106594126447No Hit
GATCCTAGAGACCATTCGCGATTCCATGAGACTCCAA460880.20149996102299617No Hit
CTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAG448840.1962359887726992No Hit
GATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGAC424230.18547632456341273No Hit
CTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC361460.1580328413282681No Hit
CTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCG284130.12422362420904334No Hit
GATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGCT274630.12007015773248013No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA266760.11662933866189563No Hit
CTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATT265310.11599538851547282No Hit
TCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGA237730.10393721952351345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA950400.030.812181
ACACGTC958700.030.66209413
TCGGAAG962650.030.5682013
ACGTCTG960700.030.54179615
CACGTCT967550.030.36414314
CGTCTGA966300.030.36138716
CACACGT968750.030.33899912
GCACACG971250.030.28198611
ATCGGAA973900.030.1992442
AGGGGGG940050.030.1981961
AGAGCAC977450.030.1041748
CGGAAGA981100.029.991514
GAGCACA981250.029.9590329
AGCACAC990750.029.7123710
CACAGTC975350.029.70899831
GAACTCC984000.029.69965221
CAGTCAC985150.029.5964227
GTCTGAA993700.029.54083317
GAAGAGC1004150.029.3713056
TCCAGTC990350.029.37110725
Produced by FastQC (version 0.11.5)