FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n01_WT_g1_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n01_WT_g1_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42510062
Sequences flagged as poor quality0
Sequence length37
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1090930.25662865417603953TruSeq Adapter, Index 2 (100% over 37bp)
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCG618220.1454290986449279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA123850.028.3272861
ACACGTC131600.026.68788513
CACCGAT132450.026.38876531
GTCACCG133600.025.9063829
ACGTCTG138100.025.45438415
TCACCGA140700.024.90745730
TCGGAAG143900.024.6780153
ATCGGAA143450.024.5177942
CGTCTGA143100.024.49994916
CACACGT147300.023.93782812
GCACACG148150.023.89461311
CACGTCT149350.023.59912514
CGACGTA38150.020.92437622
CGGAAGA172000.020.763474
AGAGCAC176450.020.3611588
AGTCACC174500.020.22516428
TATCGCG89700.019.8015299
ATCGCGG90000.019.71835110
TCGACGT40750.019.551421
GAGCACA187300.019.3800879
Produced by FastQC (version 0.11.5)