FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n01_EZH2KO_g3_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n01_EZH2KO_g3_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17894473
Sequences flagged as poor quality0
Sequence length37
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3760212.1013248057095617No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGA2863031.5999521192940414TruSeq Adapter, Index 19 (100% over 37bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1902841.0633674431205657No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG973140.5438215475806413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA311450.030.3688931
ACACGTC314600.030.19044513
AGTCACG312050.030.13038428
CACGTGA311150.030.07805631
GTCACGT314550.029.84656329
ACGTCTG317500.029.80737515
TCGGAAG320500.029.661593
CGTCTGA319150.029.65318916
CACACGT320300.029.64333512
CACGTCT320200.029.61420414
GCACACG321650.029.54783211
TCACGTG317600.029.46721330
ATCGGAA322100.029.4613932
CGGAAGA330900.028.7430744
AGAGCAC337150.028.2307058
AGCACAC341600.027.91747310
GAGCACA341750.027.896079
CAGTCAC341650.027.71532627
GAACTCC342400.027.62362521
AGGGGGG213100.027.5358661
Produced by FastQC (version 0.11.5)