FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n01_EZH2KO_g3_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n01_EZH2KO_g3_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36907920
Sequences flagged as poor quality0
Sequence length37
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG3641190.9865606081296372TruSeq Adapter, Index 12 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1262900.3421758798653514No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG564990.15308096473602414No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCG500480.1356023314237161No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG424530.11502409238992606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA391850.029.8225781
ACACGTC396650.029.56068413
ACGTCTG401050.029.20162215
CGTCTGA405100.028.89804516
TCGGAAG409750.028.6931653
GCACACG409550.028.66325211
CACACGT409650.028.618512
ATCGGAA410000.028.592692
CACGTCT412100.028.46382514
CGGAAGA428800.027.4147074
AGAGCAC436350.027.016798
AGTCACC434700.026.72054328
GAGCACA446150.026.4265359
AGCACAC446450.026.38443810
GTCACCT449550.025.76203229
GAACTCC453450.025.7162821
GTCTGAA459750.025.58696417
CAGTCAC460750.025.31769827
TCCAGTC469850.024.76513925
TGAACTC473550.024.7359720
Produced by FastQC (version 0.11.5)