FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n01_EZH2KO_g2_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n01_EZH2KO_g2_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24588020
Sequences flagged as poor quality0
Sequence length37
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7276462.9593517493478534No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCC3338631.3578279178233952TruSeq Adapter, Index 18 (100% over 37bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2172230.8834505584426888No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1148830.4672316030326964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA353700.030.2038021
ACACGTC358350.029.92143813
AGTCACG357050.029.71862228
TCACGTC357700.029.6126230
GTCACGT358600.029.56855629
ACGTCTG361900.029.53389215
TCGGAAG363900.029.4502143
CACACGT364550.029.4163912
CACGTCT363950.029.39716714
ATCGGAA363800.029.39442
GCACACG366000.029.34225711
CGTCTGA365300.029.25894516
CACGTCC363650.029.05570231
CGGAAGA378050.028.3847084
AGAGCAC385850.027.9092398
AGGGGGG251950.027.652421
AGCACAC389900.027.58350410
GAGCACA392900.027.3923919
CAGTCAC396950.026.81742127
GAACTCC398150.026.77337621
Produced by FastQC (version 0.11.5)