FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n01_EZH2KO_g2_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n01_EZH2KO_g2_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36825954
Sequences flagged as poor quality0
Sequence length37
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1449120.3935050806830422No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG746940.20282977597810503No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG515960.1401077077324324No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCG500260.1358444101678941No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA405170.11002294740280183TruSeq Adapter, Index 7 (100% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA52900.024.6165351
ACACGTC56400.023.3040913
CGACGTA47000.021.89824122
TATCGCG99550.021.408219
ATCGCGG101500.020.99689110
ACGTCTG64000.020.60927215
CGTCTGA66300.019.89421316
TCGACGT52550.019.58548521
ATATCGC110600.019.409658
CTAGTAC82350.019.3861949
CCCTACG41500.019.30928227
TCGGAAG70000.019.132013
GCACACG70150.018.97935711
GACGTAG55300.018.919923
TCGCGGC113500.018.91341211
AGGGGGG124500.018.82721
CGCTCGA55200.018.78587218
CGGCCGC116850.018.43747714
CACACGT71150.018.4292112
CACGTCT72050.018.39278814
Produced by FastQC (version 0.11.5)