FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n01_EZH2KO_g1_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n01_EZH2KO_g1_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24760515
Sequences flagged as poor quality0
Sequence length37
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11381224.596519902756465No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT6715852.7123224214035937TruSeq Adapter, Index 16 (100% over 37bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2483171.0028749402021726No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1284360.5187129589186654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA725650.030.6518631
ACACGTC730600.030.45399713
ACGTCTG735600.030.20045715
TCGGAAG741050.030.13683
ATCGGAA740500.030.102912
CACACGT739650.030.07706512
CACGTCT739150.030.07434314
CACCCGT720450.030.07197631
CGTCTGA738450.030.0561316
GCACACG740800.030.04932411
TCACCCG724150.029.96535330
CGGAAGA756600.029.5030754
AGAGCAC756850.029.4608238
GAGCACA761100.029.278169
AGCACAC762700.029.21070710
GTCACCC745400.029.18595329
AGTCACC748950.029.17799228
GAACTCC765100.028.92212121
CAGTCAC762900.028.72996127
GTCTGAA776900.028.62090517
Produced by FastQC (version 0.11.5)