FastQCFastQC Report
Sat 31 Aug 2019
HFFLGBGXC_n01_EZH2KO_g1_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFLGBGXC_n01_EZH2KO_g1_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33565195
Sequences flagged as poor quality0
Sequence length37
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1157300.3447916807871964No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA885760.2638924040214871TruSeq Adapter, Index 6 (100% over 37bp)
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCG553150.16479868506648032No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG526540.1568708300368879No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG437300.13028376566857425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA102350.028.3862421
GTCACGC104350.027.88010629
ACACGTC107250.027.37131113
AGTCACG108250.026.99019628
ACGTCTG113500.025.85030715
CGTCTGA116250.025.2253516
TCGGAAG120150.024.486433
CACACGT120200.024.46094312
GCACACG122300.024.12974711
CACGTCT124250.023.6887514
ATCGGAA123850.023.6548462
TCACGCC137600.021.60489330
CACGCCA140200.021.42537331
CGGAAGA139250.021.1500514
CGACGTA31600.020.8956322
AGAGCAC144400.020.5765728
GAGCACA156150.019.3655369
ATCGCGG85400.018.92959410
TATCGCG85700.018.9175599
CTAGTAC94450.018.7403779
Produced by FastQC (version 0.11.5)