FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n02_dad6_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n02_dad6_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20028199
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1548400.7731099536208922Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1446650.7223065838321259No Hit
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC1056290.527401390409592Illumina Single End PCR Primer 1 (100% over 49bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG752950.3759449364368708Illumina Single End PCR Primer 1 (100% over 48bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG749020.3739827030877814Illumina Single End PCR Primer 1 (100% over 48bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC745960.37245485727398653Illumina Single End PCR Primer 1 (100% over 49bp)
AAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC740210.36958390517290146Illumina Single End PCR Primer 1 (100% over 47bp)
AGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC735530.367247199810627Illumina Single End PCR Primer 1 (100% over 47bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC701620.35031607185448876Illumina Single End PCR Primer 1 (100% over 49bp)
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC694450.34673611940844007Illumina Single End PCR Primer 1 (100% over 47bp)
AAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG639760.3194296202069892Illumina Single End PCR Primer 1 (100% over 48bp)
AAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC639320.31920992995925396Illumina Single End PCR Primer 1 (100% over 47bp)
GAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC608690.30391649294077816Illumina Single End PCR Primer 1 (100% over 47bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG527410.2633337126318747Illumina Single End PCR Primer 1 (100% over 48bp)
AGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC505680.252484010169861Illumina Single End PCR Primer 1 (100% over 47bp)
GCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG504750.25201966487351163Illumina Single End PCR Primer 1 (100% over 48bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC502590.2509411854755388Illumina Single End PCR Primer 1 (100% over 49bp)
AACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC482040.2406806523142695Illumina Single End PCR Primer 1 (100% over 47bp)
AGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC480960.2401414126152831Illumina Single End PCR Primer 1 (100% over 47bp)
ACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG468120.2337304517495557Illumina Single End PCR Primer 1 (100% over 48bp)
CAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG451460.2254121800966727Illumina Single End PCR Primer 1 (100% over 48bp)
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG442520.22094847369950738Illumina Single End PCR Primer 1 (100% over 48bp)
GTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC438210.21879650786373753Illumina Single End PCR Primer 1 (100% over 47bp)
GGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC435410.2173984790145135Illumina Single End PCR Primer 1 (100% over 47bp)
GGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC432490.21594053464317983Illumina Single End PCR Primer 1 (100% over 47bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT424970.21218582859097815Illumina Single End PCR Primer 1 (100% over 46bp)
ATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC421410.2104083347683933Illumina Single End PCR Primer 1 (100% over 47bp)
TAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG421370.2103883629276901Illumina Single End PCR Primer 1 (100% over 48bp)
AGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC396640.19804077241293636Illumina Single End PCR Primer 1 (100% over 47bp)
AATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC389440.1944458410863603Illumina Single End PCR Primer 1 (100% over 47bp)
GTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG380360.18991223324673379Illumina Single End PCR Primer 1 (100% over 48bp)
GACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC379080.18927313434423135Illumina Single End PCR Primer 1 (100% over 47bp)
TAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC355710.17760458641338644Illumina Single End PCR Primer 1 (100% over 47bp)
AGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT354120.17681070574543423Illumina Single End PCR Primer 1 (100% over 46bp)
ACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC344720.1721173231801821Illumina Single End PCR Primer 1 (100% over 47bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG337640.1685823073757156Illumina Single End PCR Primer 1 (100% over 48bp)
ACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC336600.1680630395174324Illumina Single End PCR Primer 1 (100% over 47bp)
AAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT334630.16707942636279977Illumina Single End PCR Primer 1 (100% over 46bp)
TGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC333930.16672991915049376Illumina Single End PCR Primer 1 (100% over 47bp)
GCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC330160.1648475731642171Illumina Single End PCR Primer 1 (100% over 47bp)
GAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT327070.16330474846989487Illumina Single End PCR Primer 1 (100% over 46bp)
GATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC324490.16201656474453843Illumina Single End PCR Primer 1 (100% over 47bp)
ATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC312590.15607494213533626Illumina Single End PCR Primer 1 (100% over 47bp)
TAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC309580.15457206112242045Illumina Single End PCR Primer 1 (100% over 47bp)
AAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT303520.15154632725588557Illumina Single End PCR Primer 1 (100% over 46bp)
GGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG300350.14996355888015692Illumina Single End PCR Primer 1 (100% over 45bp)
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG295100.14734225478786186Illumina Single End PCR Primer 1 (100% over 48bp)
AGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT276030.137820679732611Illumina Single End PCR Primer 1 (100% over 46bp)
AAGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT259700.12966717576552939Illumina Single End PCR Primer 1 (100% over 46bp)
TGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG249280.12446451126234565Illumina Single End PCR Primer 1 (100% over 45bp)
AGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT248110.12388033492177705Illumina Single End PCR Primer 1 (100% over 46bp)
CAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC236860.1182632547240019Illumina Single End PCR Primer 1 (100% over 47bp)
AAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT236170.11791874047187167Illumina Single End PCR Primer 1 (100% over 46bp)
GTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC225920.11280095629167654Illumina Single End PCR Primer 1 (100% over 47bp)
GGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT225370.11252634348200755Illumina Single End PCR Primer 1 (100% over 46bp)
ATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC224130.11190721642020832Illumina Single End PCR Primer 1 (100% over 47bp)
GAGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT221180.1104342931683473Illumina Single End PCR Primer 1 (100% over 46bp)
GAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC219420.10955553217740646Illumina Single End PCR Primer 1 (100% over 47bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC218580.10913612352263925Illumina Single End PCR Primer 1 (98% over 50bp)
GCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC213260.10647986870911358Illumina Single End PCR Primer 1 (100% over 47bp)
CAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC212990.10634505878436698Illumina Single End PCR Primer 1 (100% over 47bp)
AGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG208920.10431292399281633Illumina Single End PCR Primer 1 (100% over 45bp)
TGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC208730.10421805774947612Illumina Single End PCR Primer 1 (100% over 47bp)
GGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT202730.10122228164399605Illumina Single End PCR Primer 1 (100% over 46bp)
AGACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT202600.10115737316171065Illumina Single End PCR Primer 1 (100% over 46bp)
TGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT202330.10102256323696404Illumina Single End PCR Primer 1 (100% over 46bp)
ACCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC201650.10068304194500964Illumina Single End PCR Primer 1 (100% over 47bp)
GTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT201020.10036848545393422Illumina Single End PCR Primer 1 (100% over 46bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)