FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n02_dad6_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n02_dad6_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15076549
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2173401.4415765836067655Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC1495700.9920705328520473Illumina Single End PCR Primer 1 (100% over 49bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC1001820.664488935763748Illumina Single End PCR Primer 1 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG731690.48531663313666806No Hit
AAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC640340.4247258440907133Illumina Single End PCR Primer 1 (100% over 47bp)
AGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC629420.4174828072392429Illumina Single End PCR Primer 1 (100% over 47bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG615440.40821012819312963Illumina Single End PCR Primer 1 (100% over 48bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG614520.40759990897121084Illumina Single End PCR Primer 1 (100% over 48bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC581920.3859769234988723Illumina Single End PCR Primer 1 (100% over 49bp)
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC571770.3792446136048773Illumina Single End PCR Primer 1 (100% over 47bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC512830.34015078649629965Illumina Single End PCR Primer 1 (100% over 49bp)
GAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC499370.3312230139669231Illumina Single End PCR Primer 1 (100% over 47bp)
AAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC469040.31110567809649275Illumina Single End PCR Primer 1 (100% over 47bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG420890.27916866121020134Illumina Single End PCR Primer 1 (100% over 48bp)
AAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG417690.27704615956874484Illumina Single End PCR Primer 1 (100% over 48bp)
AGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC392550.26037125604805184Illumina Single End PCR Primer 1 (100% over 47bp)
AACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC388410.25762526954941745Illumina Single End PCR Primer 1 (100% over 47bp)
GCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG386000.2560267605006955Illumina Single End PCR Primer 1 (100% over 48bp)
ACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG377820.2506011156797222Illumina Single End PCR Primer 1 (100% over 48bp)
AGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC361080.23949777896785265Illumina Single End PCR Primer 1 (100% over 47bp)
GGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC356510.23646658131114753Illumina Single End PCR Primer 1 (100% over 47bp)
GTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC343220.22765156668147335Illumina Single End PCR Primer 1 (100% over 47bp)
ATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC335580.22258409401249582Illumina Single End PCR Primer 1 (100% over 47bp)
CAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG335010.22220602340761136Illumina Single End PCR Primer 1 (100% over 48bp)
AGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC331690.22000392795460022Illumina Single End PCR Primer 1 (100% over 47bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT322340.21380224347096938Illumina Single End PCR Primer 1 (100% over 46bp)
AATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC313910.20821077820925732Illumina Single End PCR Primer 1 (100% over 47bp)
TAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG308630.20470865050085402Illumina Single End PCR Primer 1 (100% over 48bp)
TAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC293730.19482575223282197Illumina Single End PCR Primer 1 (100% over 47bp)
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG290650.19278284440292004Illumina Single End PCR Primer 1 (100% over 48bp)
GGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC289680.19213946109285354Illumina Single End PCR Primer 1 (100% over 47bp)
GTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG289410.19196037501685564Illumina Single End PCR Primer 1 (100% over 48bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG287100.19042819414442919Illumina Single End PCR Primer 1 (100% over 48bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC277760.18423314247842792Illumina Single End PCR Primer 1 (98% over 50bp)
GACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC277630.18414691584924375Illumina Single End PCR Primer 1 (100% over 47bp)
AGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT277630.18414691584924375Illumina Single End PCR Primer 1 (100% over 46bp)
AAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT274560.18211064083697137Illumina Single End PCR Primer 1 (100% over 46bp)
TGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC272680.18086367112261564Illumina Single End PCR Primer 1 (100% over 47bp)
GAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT262730.1742640175812117Illumina Single End PCR Primer 1 (100% over 46bp)
ACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC260420.17273183670878528Illumina Single End PCR Primer 1 (100% over 47bp)
GATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC257140.17055627252629232Illumina Single End PCR Primer 1 (100% over 47bp)
AAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT251400.16674903520692966Illumina Single End PCR Primer 1 (100% over 46bp)
ACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC248430.1647790883709528Illumina Single End PCR Primer 1 (100% over 47bp)
GCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC228790.15175223454651326Illumina Single End PCR Primer 1 (100% over 47bp)
ATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC227290.1507573119020805Illumina Single End PCR Primer 1 (100% over 47bp)
AGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT223510.14825010683810996Illumina Single End PCR Primer 1 (100% over 46bp)
TAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC222600.14764652043382076Illumina Single End PCR Primer 1 (100% over 47bp)
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG204600.1357074487006277Illumina Single End PCR Primer 1 (100% over 48bp)
GGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG204200.13544213599544563Illumina Single End PCR Primer 1 (100% over 45bp)
AAGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT187340.12425920547202148Illumina Single End PCR Primer 1 (100% over 46bp)
AAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT180820.11993460837755378Illumina Single End PCR Primer 1 (100% over 46bp)
TGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG176580.11712229370262385Illumina Single End PCR Primer 1 (100% over 45bp)
AGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT171530.11377272079970024Illumina Single End PCR Primer 1 (100% over 46bp)
CCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG167160.11087417949558617Illumina Single End PCR Primer 1 (100% over 48bp)
ATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC166750.11060223397277454Illumina Single End PCR Primer 1 (100% over 47bp)
CAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC165660.10987925685115339Illumina Single End PCR Primer 1 (100% over 47bp)
GTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC164810.1093154673526415Illumina Single End PCR Primer 1 (100% over 47bp)
CAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC162360.10769042703340134Illumina Single End PCR Primer 1 (100% over 47bp)
GAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC162190.10757766913369897Illumina Single End PCR Primer 1 (100% over 47bp)
GAGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT162150.10755113786318077Illumina Single End PCR Primer 1 (100% over 46bp)
GGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT161670.10723276261696227Illumina Single End PCR Primer 1 (100% over 46bp)
GGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT158560.1051699563341717Illumina Single End PCR Primer 1 (100% over 46bp)
GCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC157440.10442708075966191Illumina Single End PCR Primer 1 (100% over 47bp)
GTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT156720.10394951789033419Illumina Single End PCR Primer 1 (100% over 46bp)
ACCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC154710.1026163215467943Illumina Single End PCR Primer 1 (100% over 47bp)
GTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC154290.10233774320635312Illumina Single End PCR Primer 1 (100% over 47bp)
AGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG153790.10200610232487554Illumina Single End PCR Primer 1 (100% over 45bp)
AGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT153650.1019132428780618Illumina Single End PCR Primer 1 (100% over 46bp)
TGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC153230.10163466453762064Illumina Single End PCR Primer 1 (100% over 47bp)
AGACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT152200.10095148432177682Illumina Single End PCR Primer 1 (100% over 46bp)
TGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT151420.10043412454667179Illumina Single End PCR Primer 1 (100% over 46bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)