FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n02_dad6_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n02_dad6_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2839674
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG577372.033226349221777No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC109140.3843398925369602Illumina Single End PCR Primer 1 (100% over 50bp)
AAAGGTTGTTTTATAGTAGTTAGGATGTAGAAAATGTATTCCGATAGGCC83040.29242793362900105No Hit
GTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC60200.21199616575705518Illumina Single End PCR Primer 1 (100% over 38bp)
GTAGGGAAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC56670.19956516135302854Illumina Single End PCR Primer 1 (100% over 40bp)
GGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGC52840.18607769765120924No Hit
GCTGAGATTAAGCCTTTGTTGTCTGATTTGTTTTTTATTTCTTTCTAAGT48000.16903348764682144No Hit
GGGTGGAGGACAATTTTTATTATATTTCATCTAATAGCAATAGGATATGA46410.16343425336852047No Hit
GTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG46070.16223693283102214Illumina Single End PCR Primer 1 (100% over 35bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC44870.1580110956398516Illumina Single End PCR Primer 1 (100% over 49bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC40120.14128382342480159Illumina Single End PCR Primer 1 (100% over 49bp)
GTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA31110.10955482918109614Illumina Single End PCR Primer 1 (100% over 36bp)
GGTGGCAGGCATAGTAAAATGATGGTGTGGAAGACATAGATGGTATTTGT30260.10656152783735034No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)