FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n02_dad6_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n02_dad6_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3054859
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG598211.958224585815581No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC125230.4099370871126949Illumina Single End PCR Primer 1 (100% over 50bp)
AAAGGTTGTTTTATAGTAGTTAGGATGTAGAAAATGTATTCCGATAGGCC92400.3024689519221673No Hit
GGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGC69550.22767008231803823No Hit
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC50970.16684894458303967Illumina Single End PCR Primer 1 (100% over 49bp)
GCTGAGATTAAGCCTTTGTTGTCTGATTTGTTTTTTATTTCTTTCTAAGT45660.14946680026803202No Hit
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC45080.14756818563475435Illumina Single End PCR Primer 1 (100% over 49bp)
TGGGGATTGTGATTCATGCTTATGTATTGGAAGAAGAGGTACACGAGTTA42640.1395809102809655No Hit
GGGTGGAGGACAATTTTTATTATATTTCATCTAATAGCAATAGGATATGA37780.12367182904350087No Hit
GTAGGGAAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC34850.11408055167194296Illumina Single End PCR Primer 1 (100% over 40bp)
GTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC34540.1130657748851911Illumina Single End PCR Primer 1 (100% over 38bp)
TGTGGGTAGGGGAATCTCGCATTTCACTGGGCCAGCATCAGTTTTGGTGG30740.1006265755637167No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)