FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n02_dad6_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n02_dad6_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3614618
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG901262.4933755102198902No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC229020.633593923341277Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC106950.29588188848724817Illumina Single End PCR Primer 1 (100% over 49bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC93100.2575652530917513Illumina Single End PCR Primer 1 (100% over 49bp)
AAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC92590.25615431561509405Illumina Single End PCR Primer 1 (100% over 47bp)
AGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC90170.24945927896115164Illumina Single End PCR Primer 1 (100% over 47bp)
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC81010.22411773526275802Illumina Single End PCR Primer 1 (100% over 47bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC80940.22392407717772667Illumina Single End PCR Primer 1 (100% over 49bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG80820.22359209188910142Illumina Single End PCR Primer 1 (100% over 48bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG79580.2201615772399739Illumina Single End PCR Primer 1 (100% over 48bp)
AAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC71960.19908051141227096Illumina Single End PCR Primer 1 (100% over 47bp)
GAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC70750.19573299308529973Illumina Single End PCR Primer 1 (100% over 47bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC67420.18652040132594924Illumina Single End PCR Primer 1 (100% over 49bp)
AAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG65840.18214926169238355Illumina Single End PCR Primer 1 (100% over 48bp)
GCTGAGATTAAGCCTTTGTTGTCTGATTTGTTTTTTATTTCTTTCTAAGT63850.17664383898934824No Hit
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG59270.16397306714015147Illumina Single End PCR Primer 1 (100% over 48bp)
AGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC58250.16115119218683688Illumina Single End PCR Primer 1 (100% over 47bp)
AAAGGTTGTTTTATAGTAGTTAGGATGTAGAAAATGTATTCCGATAGGCC58120.1607915414574929No Hit
GCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG55460.1534325342263Illumina Single End PCR Primer 1 (100% over 48bp)
AGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC54820.1516619460202987Illumina Single End PCR Primer 1 (100% over 47bp)
AACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC54740.15144062249454854Illumina Single End PCR Primer 1 (100% over 47bp)
ACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG54270.15014034678076632Illumina Single End PCR Primer 1 (100% over 48bp)
TGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACTGAAGTGGGG53700.14856341665979642No Hit
GGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC51450.1423386924980731Illumina Single End PCR Primer 1 (100% over 47bp)
GTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC51170.14156406015794754Illumina Single End PCR Primer 1 (100% over 47bp)
ATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC48300.13362407867166046Illumina Single End PCR Primer 1 (100% over 47bp)
AGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC47660.13185349046565917Illumina Single End PCR Primer 1 (100% over 47bp)
CAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG47410.1311618544476899Illumina Single End PCR Primer 1 (100% over 48bp)
TAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG47170.13049788387043942Illumina Single End PCR Primer 1 (100% over 48bp)
AATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC47170.13049788387043942Illumina Single End PCR Primer 1 (100% over 47bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT45370.12551810454106077Illumina Single End PCR Primer 1 (100% over 46bp)
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG44350.12269622958774619Illumina Single End PCR Primer 1 (100% over 48bp)
GGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC43030.11904439141286853Illumina Single End PCR Primer 1 (100% over 47bp)
GTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG42870.1186017443613682Illumina Single End PCR Primer 1 (100% over 48bp)
TAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC41860.1158075348487724Illumina Single End PCR Primer 1 (100% over 47bp)
GACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC40430.11185137682598825Illumina Single End PCR Primer 1 (100% over 47bp)
AGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT39270.10864218570261089Illumina Single End PCR Primer 1 (100% over 46bp)
TGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC38870.1075355680738601Illumina Single End PCR Primer 1 (100% over 47bp)
AAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT38090.10537766369779601Illumina Single End PCR Primer 1 (100% over 46bp)
GATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC37790.1045477004762329Illumina Single End PCR Primer 1 (100% over 47bp)
ACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC37390.10344108284748207Illumina Single End PCR Primer 1 (100% over 47bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG37280.10313676299957561Illumina Single End PCR Primer 1 (100% over 48bp)
GAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT37200.10291543947382545Illumina Single End PCR Primer 1 (100% over 46bp)
GGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGC36820.10186415272651218No Hit
ACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC36780.1017534909636371Illumina Single End PCR Primer 1 (100% over 47bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)