FastQCFastQC Report
Fri 3 Mar 2023
HFFF7AFX5_n02_dad6_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFF7AFX5_n02_dad6_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12421634
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1576781.2693821118864073No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1422831.1454451161578258Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC600660.483559570343161Illumina Single End PCR Primer 1 (100% over 49bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC511670.4119184319872893Illumina Single End PCR Primer 1 (100% over 49bp)
AAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC312930.2519233781964595Illumina Single End PCR Primer 1 (100% over 47bp)
AGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC305510.2459499289706974Illumina Single End PCR Primer 1 (100% over 47bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG295130.23759354043115422Illumina Single End PCR Primer 1 (100% over 48bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC292460.23544406476635843Illumina Single End PCR Primer 1 (100% over 49bp)
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC281260.22642753763313264Illumina Single End PCR Primer 1 (100% over 47bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG280590.22588815609927002Illumina Single End PCR Primer 1 (100% over 48bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC277390.22331200548977695Illumina Single End PCR Primer 1 (100% over 49bp)
GAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC246520.19846020257882338Illumina Single End PCR Primer 1 (100% over 47bp)
AAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC223740.18012123042749448Illumina Single End PCR Primer 1 (100% over 47bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG200580.1614763403912883Illumina Single End PCR Primer 1 (100% over 48bp)
AAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG196840.15846546436644326Illumina Single End PCR Primer 1 (100% over 48bp)
AACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC180850.1455927617896325Illumina Single End PCR Primer 1 (100% over 47bp)
AGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC177150.14261408764740613Illumina Single End PCR Primer 1 (100% over 47bp)
GTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC175200.1410442458697463Illumina Single End PCR Primer 1 (100% over 47bp)
GCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG174250.14027945115755303Illumina Single End PCR Primer 1 (100% over 48bp)
AGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC173560.13972396868238107Illumina Single End PCR Primer 1 (100% over 47bp)
GGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC172420.13880621502774917Illumina Single End PCR Primer 1 (100% over 47bp)
ACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG170790.13749398831103862Illumina Single End PCR Primer 1 (100% over 48bp)
AGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC161830.130280766604458Illumina Single End PCR Primer 1 (100% over 47bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC159140.1281151899983529Illumina Single End PCR Primer 1 (98% over 50bp)
AATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC158510.12760801034710892Illumina Single End PCR Primer 1 (100% over 47bp)
ATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC157580.126859316576225Illumina Single End PCR Primer 1 (100% over 47bp)
CAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG152620.12286628313151071Illumina Single End PCR Primer 1 (100% over 48bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT149400.1202740315807083Illumina Single End PCR Primer 1 (100% over 46bp)
TAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG142540.11475140871160751Illumina Single End PCR Primer 1 (100% over 48bp)
TGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACTGAAGTGGGG141400.1138336550569756No Hit
TAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC141370.11380950364501159Illumina Single End PCR Primer 1 (100% over 47bp)
GTAGGGAAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC139410.11223161139669707Illumina Single End PCR Primer 1 (100% over 40bp)
AGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT136390.10980036925898799Illumina Single End PCR Primer 1 (100% over 46bp)
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG135330.1089470193695934Illumina Single End PCR Primer 1 (100% over 48bp)
GTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG133990.10786825630186817Illumina Single End PCR Primer 1 (100% over 48bp)
GCTGAGATTAAGCCTTTGTTGTCTGATTTGTTTTTTATTTCTTTCTAAGT130720.10523575239779243No Hit
AAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT130340.10492983451291513Illumina Single End PCR Primer 1 (100% over 46bp)
AAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT126580.10190285754676076Illumina Single End PCR Primer 1 (100% over 46bp)
GACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC125580.10109781048129415Illumina Single End PCR Primer 1 (100% over 47bp)
GATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC125420.10096900295081951Illumina Single End PCR Primer 1 (100% over 47bp)
GGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC125380.10093680106820085Illumina Single End PCR Primer 1 (100% over 47bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)